Hangzhou Kanlong Imp&Exp Co., Ltd.

Hangzhou Kanlong Imp&Exp Co., Ltd.

Zhejiang,China

Hangzhou Kanlong Imp&Exp Co., Ltd.

Zhejiang,China

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Hangzhou Kanlong Imp&Exp Co., Ltd.
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Hangzhou Kanlong Imp&Exp Co., Ltd.

Zhejiang,China

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Rapid Test COVID-19 IgG/IgM combo test cassette

FOB Price : Get a Price/Quote

Min.Order : 1000 Piece(s)

Certification : CFDA,GMP,ISO13485,FDA

Brand Name : N/A

Payment Terms : T/T

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  • brand name : N/A

  • certification : CFDA,GMP,ISO13485,FDA

  • min.order : 1000 Piece(s)

  • warranty :

  • payment terms : T/T

  • Packaging :

  • Specification :

  • place of origin : China

product description

Model:Cov-19                                                                               Certification: /

Dimension: 4.0mm cassette                                                         Packaging Details: foil bag

Brand Name: N/A                                                                         Place of Origin: China
  
Warranty: 1 year                                                                           Color: white

Size: /                                                                                            Finish:/

Customer's Logo: /                                                                       MOQ: 1000 kits

Processing Time: 1000 kits 2 days                                               Usage: diagnostic the coronavirus

Quality: /

Packing:/

Development of Novel coronavirus antibody test kit

There are three types of Novel coronavirus antibody test kit : 1,IgG/IgM three lines test kit ; 2,IgM/Total antibody three-line test kit ; 3,IgG antibody and IgM antibody two-lines test kit.
The test platform including Immunochromatography,ELISA and CLIA.

1.Immunochromatography test platform:
The immune tomography detection platform has the advantages of fast reading speed, simple operation, low professional requirements for operators, and not strict environmental requirements for detection, so it is currently the most important application platform for POCT detection. According to the different markers, mainly include colloidal gold, latex, immunofluorescence and other subdivision areas. The application of N protein and S protein are mainly divided into two types: marker and envelope.

1.1 Conjugate of N/S protein :
Biomapper has developed the high and stable expression of N/S protein, which could be stably preserved in various buffers .In conjugating of colloidal gold and latex,conventional PB, Tris-cl, PBS can all be used, and the requirements for gold particles are also more flexible. The isoelectric point of N/S protein is high, but it can be stable in neutral preservation solution, so the process of labeling is not high, so it can be used as conventional Conjugate. At the same time, because the protein is stable, and the structure of the protein is stable in the process of Conjugating, the effect on the protein performance is too small. can be developed and applied using the pattern of Conjugating N/S protein, coated with mouse anti-human IgG/IgM.


Fig3:1ml Cojugate and 4ml Conjugate of Novel Coronavirus

1.2 The Coating of N/S protein
The protein belongs to soluble protein, the stability of the protein has a great influence on the activity of the protein, and the proper preservation solution and coating are more important for the display of antigenic properties. The N/S protein developed by Mai Yue can be stable in many kinds of preservation solution, so it is convenient for coating, and the technology of Conjugating mouse anti-human IgG and IgM is more mature.

Fig4:Coating of Covid-19 Antigen

1.3 The combination of N protein and S protein can improve the accuracy of new coronavirus.
N proteins are highly conserved from other coronaviruses, but S proteins differ from fragments of other coronaviruses, so the use of the two proteins is crucial for the accuracy of the tests.


Fig5:Covid-19 IgG/IgM Uncut sheets and cassttes

2. ELISA platform
The traditional ELISA platform has high sensitivity and high sample size, which has played a key role since the beginning of the development of the IVD industry. The N/S protein is mainly used in coating and conjugating HRP.
2.1 The coat of N/S portein-HRP:
In the process of Conjugating HRP, the routine use of PB buffer, then the stability of antigens in PB buffer is crucial. The N/S protein developed by Maiyue is highly stable in PB buffer and can be applied to the screening of Conjugating HRP for the detection of novel coronavirus iGm antibodies.
2.2 The coat of N/S protein:
It can be directly use Tris-Cl, PB and other buffers coat enzyme labeled plates for detection of new coronavirus IgG antibodies in screening.
 
3. CLIA
The commonly used Conjugate of CLIA platform are AP enzyme, acridine ester and so on. At present, theindicators of new coronavirus screening are IgG and IgM. Because IgM antibody is produced in the early stage of new coronavirus infection, the serum content is relatively small, so IgM antibody of new coronavirus in human serum can be detected by capture method or indirect method.
3.1 Detection for IgM antibody:
Capture method: Magnetic beads coats mouse anti-human IgM antibody, N/S protein Conjugates AP enzyme oracridine ester. Commonly used buffer systems are PB and CB. Indirect method: Magnetic bead coated N/S protein, mouse anti-human IgM Conjugating AP enzyme or acridine ester.
3.2 Detection for IgG antibody
IgG antibody is produced relatively late , and the serum content is relatively high, so IgG antibody of new coronavirus in human serum can be detected directly by Indirect method. N/S proteins coat magnetic beads, and anti-human IgG antibodies conjugate AP enzymes or acridine esters.


 
 
Rapid Test COVID-19 IgG/IgM combo test cassette
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product information

product description

Model:Cov-19                                                                               Certification: /

Dimension: 4.0mm cassette                                                         Packaging Details: foil bag

Brand Name: N/A                                                                         Place of Origin: China
  
Warranty: 1 year                                                                           Color: white

Size: /                                                                                            Finish:/

Customer's Logo: /                                                                       MOQ: 1000 kits

Processing Time: 1000 kits 2 days                                               Usage: diagnostic the coronavirus

Quality: /

Packing:/

Development of Novel coronavirus antibody test kit

There are three types of Novel coronavirus antibody test kit : 1,IgG/IgM three lines test kit ; 2,IgM/Total antibody three-line test kit ; 3,IgG antibody and IgM antibody two-lines test kit.
The test platform including Immunochromatography,ELISA and CLIA.

1.Immunochromatography test platform:
The immune tomography detection platform has the advantages of fast reading speed, simple operation, low professional requirements for operators, and not strict environmental requirements for detection, so it is currently the most important application platform for POCT detection. According to the different markers, mainly include colloidal gold, latex, immunofluorescence and other subdivision areas. The application of N protein and S protein are mainly divided into two types: marker and envelope.

1.1 Conjugate of N/S protein :
Biomapper has developed the high and stable expression of N/S protein, which could be stably preserved in various buffers .In conjugating of colloidal gold and latex,conventional PB, Tris-cl, PBS can all be used, and the requirements for gold particles are also more flexible. The isoelectric point of N/S protein is high, but it can be stable in neutral preservation solution, so the process of labeling is not high, so it can be used as conventional Conjugate. At the same time, because the protein is stable, and the structure of the protein is stable in the process of Conjugating, the effect on the protein performance is too small. can be developed and applied using the pattern of Conjugating N/S protein, coated with mouse anti-human IgG/IgM.


Fig3:1ml Cojugate and 4ml Conjugate of Novel Coronavirus

1.2 The Coating of N/S protein
The protein belongs to soluble protein, the stability of the protein has a great influence on the activity of the protein, and the proper preservation solution and coating are more important for the display of antigenic properties. The N/S protein developed by Mai Yue can be stable in many kinds of preservation solution, so it is convenient for coating, and the technology of Conjugating mouse anti-human IgG and IgM is more mature.

Fig4:Coating of Covid-19 Antigen

1.3 The combination of N protein and S protein can improve the accuracy of new coronavirus.
N proteins are highly conserved from other coronaviruses, but S proteins differ from fragments of other coronaviruses, so the use of the two proteins is crucial for the accuracy of the tests.


Fig5:Covid-19 IgG/IgM Uncut sheets and cassttes

2. ELISA platform
The traditional ELISA platform has high sensitivity and high sample size, which has played a key role since the beginning of the development of the IVD industry. The N/S protein is mainly used in coating and conjugating HRP.
2.1 The coat of N/S portein-HRP:
In the process of Conjugating HRP, the routine use of PB buffer, then the stability of antigens in PB buffer is crucial. The N/S protein developed by Maiyue is highly stable in PB buffer and can be applied to the screening of Conjugating HRP for the detection of novel coronavirus iGm antibodies.
2.2 The coat of N/S protein:
It can be directly use Tris-Cl, PB and other buffers coat enzyme labeled plates for detection of new coronavirus IgG antibodies in screening.
 
3. CLIA
The commonly used Conjugate of CLIA platform are AP enzyme, acridine ester and so on. At present, theindicators of new coronavirus screening are IgG and IgM. Because IgM antibody is produced in the early stage of new coronavirus infection, the serum content is relatively small, so IgM antibody of new coronavirus in human serum can be detected by capture method or indirect method.
3.1 Detection for IgM antibody:
Capture method: Magnetic beads coats mouse anti-human IgM antibody, N/S protein Conjugates AP enzyme oracridine ester. Commonly used buffer systems are PB and CB. Indirect method: Magnetic bead coated N/S protein, mouse anti-human IgM Conjugating AP enzyme or acridine ester.
3.2 Detection for IgG antibody
IgG antibody is produced relatively late , and the serum content is relatively high, so IgG antibody of new coronavirus in human serum can be detected directly by Indirect method. N/S proteins coat magnetic beads, and anti-human IgG antibodies conjugate AP enzymes or acridine esters.


 
 

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